AgentSkillsCN

PETase 2024–2025 Literature Scan

使用PubMed & Scholar Gateway从2024–2025年的论文中提取PETase（和PET水解酶）变体及效应，生成带引用的标准表格。

SKILL.md
--- frontmatter
name: "PETase 2024–2025 Literature Scan"
description: "Use PubMed & Scholar Gateway to extract PETase (and PET hydrolase) variants and effects from 2024–2025 papers into a standardized table with citations."
version: "1.0.0"

Goal

Create a 2024–2025 PETase variants & effects evidence table using PubMed and Scholar Gateway connectors, with strict, citable provenance.

When to use

  • •The user asks for PETase or PET hydrolase variant scouting, benchmarking, or zero-shot hypothesis generation focused on 2024–2025.

Operating rules

  1. •Always use connectors (PubMed first; Scholar Gateway for Wiley/paywalled content). Prefer PMC for full text when available.
  2. •Restrict the search window to 2024-01-01 .. 2025-10-21.
  3. •For each paper, extract mutations, assay comparability, performance endpoints, thermostability, expression notes, rationale, and citations.
  4. •Deduplicate by DOI; include PMID/PMCID when present.
  5. •Use the Output schema exactly (see resources/output-schema.csv).

Query seeds

(See resources/query-strings.md for adaptable queries.)

Output format

  • •Primary: table matching the schema (CSV in a fenced code block).
  • •Secondary: ranked shortlist (5–10 variants) + zero-shot hypotheses.

Steps

  1. •Search PubMed with Boolean + fielded queries; filter 2024–2025; sort by date.
  2. •For promising records, fetch abstracts/full text (PMC if available). Cross-check in Scholar Gateway to access Wiley content.
  3. •Extract data into the schema; convert/standardize units carefully.
  4. •Deduplicate by DOI; verify all rows have at least one citation (PMID/PMCID/DOI).
  5. •Produce the CSV table, then a ranked shortlist and 2–3 hypotheses.

Safety & provenance

  • •Cite rigorously (PMID/PMCID/DOI). No hallucinated values.
  • •If a metric is missing, leave it blank.
  • •Do not train or store proprietary datasets; this is a literature-only workflow suitable for Zero‑Shot reasoning.