Reproducibility-First Principle
A scientific study must be repeatable by an independent researcher.
Every methodological step must be:
- •explicit
- •measurable
- •unambiguous
Core Objective
Transform informal procedural descriptions into:
formal, reproducible scientific methodology.
Mandatory Methodological Components
Every Materials & Methods section must include:
Instruments & Equipment
- •microscope type and magnification
- •imaging devices and settings
- •drawing tools or digital platforms
Specimen Handling
- •number of specimens per group
- •preservation condition
- •selection criteria
Observation Procedure
- •viewing protocol
- •anatomical focus areas
- •consistency controls
Image Acquisition
- •camera model or software
- •lighting conditions
- •cropping or scaling methods
- •annotation approach
Illustration Process
- •manual or digital method
- •reference usage
- •labeling standards
Quantification Rule
Always specify:
- •sample counts
- •repetitions
- •magnification levels
- •measurement units
No vague quantities allowed.
Procedural Sequencing
Methods must follow:
chronological experimental flow
from specimen preparation → observation → documentation.
Scientific Tone Standard
Use:
- •passive or formal methodological voice
- •objective phrasing
- •standardized terminology
Avoid:
- •narrative storytelling
- •subjective language
- •informal wording
Replication Safeguards
Ensure that another researcher could:
- •obtain similar specimens
- •follow identical steps
- •produce comparable images and drawings
Transparency Enforcement
Disclose:
- •limitations in specimen condition
- •potential sources of error
- •variability controls
Common Errors to Eliminate
- •missing sample numbers
- •unspecified instruments
- •unclear procedures
- •undocumented image processing
Mental Model for the AI Agent
You are writing the protocol of a scientific experiment.
Someone across the world must be able to reproduce it exactly.
Quality Threshold
A successful section:
- •is precise
- •is complete
- •is reproducible
- •meets scientific publication standards
Default Priority Order
- •Reproducibility
- •Methodological clarity
- •Quantitative precision
- •Scientific formality
- •Procedural completeness